Sunday, December 9, 2018

Adding metalurgical capability to a biological microscope - part 1

Biological microscopes among a few other things, are characterized by the fact that rely on transmitted light rather than reflected light, to produce an image of the specimen.This is adequate for most biological needs because the specimen can either be made thin enough for light to go through, or the medium and/or the specimen itself is relatively transparent to light.

However there are other applications in microscopy where it cannot be assumed that the specimen is transparent or able to be ground to a thickness small enough for light to traverse it. Some materials are opaque even at thicknesses of few microns.

The only solution in these cases is to rely on reflected light. For this to be possible, a different illumination system is required on a microscope. This type of illumination is commonly designated of epi-illumination (as opposed to trans-illumination used for biological observations). This is where a device known as vertical illuminator comes into play (picture obtained from a Olympus BHM manual):